Practical – I (Lower Cryptogams and Cell Biology)

BOT: DSC-152 Course Title: Practical — I (Lower Cryptogams and cell Biology) Credits: 3 Contact hours: 60 Marks: 100 (All units are of equal credits) Course objective: To study the cellular organisation of lower cryptogams and other eukaryotes Unit-I: Microbes Models of virus — T Phage, Lytic & lysogenic cycle from photograph Study of bacterial reproduction- binary fission, Conjugation and endospores from temporary/permanent slides/ photographs. Gram staining techniques in bacteria through Curd/ Root nodules. Preparation of media- nutrient agar and broth Unit-II: Algae Identification and study of vegetative / reproductive structures of the following genera through temporary preparation of slides / permanent slides — Cyanobacteria: Nostoc / Anabaena Chlorophyceae: Chlamydomonas, Chlorella, Volvox and Chara; Xanthophyceae: Voucheria; Bacillariophyceae: Pinnularia; Phaeophyceae: Ectocarpus / Sargassum; Rhodophyceae: Polysiphonia / Batrachospermum. Unit-I: Fungi & Lichen 14

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Identification and study of vegetative / reproductive structures of the following genera through temporary preparation of slides (by Lactophenol Cotton Blue methods) – Rhizopus, Penicillium, Ustilago and Puccinia. Study of Crustose, Foliose and Fruticose Lichen.

Unit-IV: Cell Biology

Study of plant cell with the help of epidermal peel mount of Onion / Rhoeo / Crinum
Cytochemical staining of DNA-feulgen and cell wall in the epidermal peel of onion using Periodic Schiff’s (PAS) staining technique. Measurement of cell size by the technique of micrometry. Counting the cells per unit volume with the help of haemocytometer. Study of phenomenon of plasmolysis and deplasmolysis Study of effect of organic solvent and temperature on membrane permeability.
Study different stages of Mitosis and Meiosis.

Unit-V: Viva / Practical Record Books / Field study.

Botanical Excursion: visit to institute of microbial technology / visit to local vegetation to understood about lower cryptogams and submission of excursion report. Viva-voce covering entire syllabus by External Examiners Submission of Practical Record Books. Course outcome: The course will provide a detailed picture of cellular organisation of lower cryptogam and eukaryotes.