Cell Biology

BOT: DSC-151 Course Title: Cell Biology Credits: 3 Contact hours: 45 Marks: 100 (All units are of equal credits) Course objective: To study the cellular organisation of prokaryotic and eukaryotic organisms Unit-I: Cell: Historical background of Cell Biology; Broad Classification of Cell Types (in Prokaryotes and Eukaryotes); Cell as basic unit of life; Cell Theory; Pre-cellular evolution and artificial creation of cell; Characteristic features of cell types: Mycoplasma (PPLO); Viroids; Prions; Archaebacteria and Eubacteria; Eukaryotic microbes; Ecological amplitude of cell in high altitude, arctic, hotspring, arid, brakish and fresh water. Unit-II: Structure and function of cell organelles: Cell wall and cell membrane; Models of cell membrane; role of various membrane proteins, lipids and carbohydrates; role of channels and pumps in cellular transport and signaling; Cytoskeleton and Cytosol; Golgi-bodies; Endoplasmic reticulum; Ribosomes; Lysosomes; Peroxysomes; Endosymbiotic Theory: Mitochondria and Chloroplast. Unit-IIl: Ultrastructure and function of Nucleus: composition of nucleus; Nucleic acids: DNA and RNA-composition, structure of DNA; A, B and Z forms of DNA; Replication of DNA, Denaturation of DNA, DNA polymerases; Different types of RNA and their role. Unit-IV: Cell Division and its regulation: Cell cycle; cell-cell interaction; cell locomotion (amoeboid, flagellar and cilliar), Muscle and nerve cell; Cell Senescence and Programmed Cell Death; Apoptosis; Cell differentiation; Biology of cancer; Cell Division: Mechanisms of Mitosis and Meiosis; Role of Centromere, Kinetochore and Spindle apparatus. Unit-V: Techniques in cell biology: Microscopy: Principles of Light and Electron microscopy - TEM and SEM; Phase contrast and fluorescence microscopy; Principles of Chromatographic techniques- Paper chromatography, TLC, Column chromatography; HPLC; Autoradiography and its applications; Centrifugation. 13

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Course Outcome: The course will provide a detailed understanding of cellular organisation of prokaryotic and eukaryotic organism. Suggested Readings

1. Campbell, MK (2012) Biochemistry, 7th ed., Published by Cengage Learning
2. Campbell, PN and Smith AD (2011) Biochemistry Illustrated, 4th ed., Published by Churchill Livingstone
3. Tymoczko JL, Berg JM and Stryer L (2012) Biochemistry: A short course, 2nd ed., W.H.Freeman
4. Berg JM, Tymoczko JL and Stryer L (2011) Biochemistry, W.H.Freeman and Company
5. Nelson DL and Cox MM (2008) Lehninger Principles of Biochemistry, 5th Edition., W.H. Freeman and Company.
6. Karp, G. (2010). Cell Biology, John Wiley & Sons, U.S.A. 6th edition.
7. Hardin, J., Becker, G., Skliensmith, L.J. (2012). Becker’s World of the Cell, Pearson Education Inc. U.S.A. 8th edition.
8. Cooper, G.M. and Hausman, R.E. (2009) The Cell: A Molecular Approach. 5th edition. ASM Press & Sunderland, Washington, D.C.; Sinauer Associates, MA.
9. Becker, W.M., Kleinsmith, L.J., Hardin. J. and Bertoni, G. P. (2009) The World of the Cell. 7th edition. Pearson Benjamin Cummings Publishing, San Francisco